Fig. 2

TLR7 is expressed by support cells in guinea pig vagal and dorsal root ganglia. Guinea pig-specific in situ hybridization probe detected TLR7 (pink) in small cells located outside the neuronal cell bodies (N) in the dorsal root ganglia and in the nodose and jugular ganglia, which comprise the vagal ganglia. The housekeeping gene Ppib (pink) was also detected in the spleen and ganglia, while the negative control probe (DapB) showed no signal as expected. Rarely, a single TLR7 in situ probe signal could be found in a neuronal cell body (N*). Representative neuronal cell bodies (N) within the ganglia are outlined in the image. Blue = hematoxylin nuclear stain in all images. N = 4, representative images are shown. Scale bar = 50 μm