Skip to main content
Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Autophagy protein NRBF2 attenuates endoplasmic reticulum stress-associated neuroinflammation and oxidative stress via promoting autophagosome maturation by interacting with Rab7 after SAH

Fig. 5

Pharmacological inhibition of Rab7 abolished the neuroprotective effect of NRBF2 against SAH at 24 h. A The lysates from cortex tissue were immunoprecipitated with anti-NRBF2. Then, immunoprecipitates were analyzed by western blotting with anti-NRBF2 and anti-Rab7. n = 6. B Immunostaining for NRBF2 and Rab7 in left cortex area. n = 3. C, D Quantification of neurological function with two different scoring systems at 24 after SAH. n = 17. E Quantification of brain water content. n = 6. F Representative microphotographs of immunofluorescence double staining showing the localization of LC3B (red) and Lamp2 (green). Scale bar = 50 μm. n = 5. (G-O) Representative western blotting images and quantitative analyses of LC3 II/I, Lamp2, CHOP, Romo-1, TXNIP, NLRP3, TNF-α, and IL-1β expression at 24 h after SAH. n = 6. Data are represented as mean ± SD (E, H–O) or median (interquartile range) (C, D). *P < 0.05 versus sham group. #P < 0.05 versus SAH + NC-AAV group. &P < 0.05 versus SAH+NRBF2-AAV + vehicle group

Back to article page

Journal of Neuroinflammation

ISSN: 1742-2094