Fig. 2

Examination of C3-positive A1 astrocytes following SCI. a–c Western blot analysis of C3 (a, b) and S100A10 (a, c) protein levels following spinal cord contusion at 0 day, 1 day, 4 days, and 7 days, respectively. Quantities were normalized to endogenous β-actin. n = 6. Experiments were performed in triplicates. Error bars represent the standard deviation, *P < 0.05, one-way ANOVA with Dunnett’s post hoc test. For C3 quantitative analysis, F (3, 8) = 15.66, P = 0.0010. 1 day vs 0 day, P = 0.0018; 4 day vs 0 day, P = 0.0024. For S100A10 quantitative analysis, F (3, 8) = 7.930, P = 0.0088. 4 day vs 0 day, P = 0.0305; 7 days vs 0 day, P = 0.0233. d Examination of C3-positive A1 astrocytes in the cross sections of rat contused spinal cord at 0 day, 1 day, 4 days, and 7 days, respectively. Rectangles indicate the region magnified. Arrowheads indicate C3-positive astrocytes. Scale bars, 200 μm or 50 μm in magnification