Fig. 8From: HSF1 is involved in suppressing A1 phenotype conversion of astrocytes following spinal cord injury in ratsWestern blot analysis of C3 and HSP70 protein levels in the injured cord. The rats were received 100 μl intraperitoneal injections of 20 mg/kg 17-AAG three times a week on alternate days. a–c Western blot analysis of C3 (a, b) and HSP70 (a, c) protein levels at 0 day, 1 day, 4 days, and 7 days following spinal cord injury. Quantities were normalized to endogenous β-actin. n = 6. Experiments were performed in triplicates. Error bars represent the standard deviation, *P < 0.05, #P < 0.05, two-way ANOVA with Tukey’s test. For C3 quantitative analysis, F (3, 6) = 78.80, P < 0.0001. 1 day vehicle vs 0 day, P < 0.0001; 4 days vehicle vs 0 day, P < 0.0001; 1 day 17-AAG vs 1 day vehicle, P = 0.0028; 4 days 17-AAG vs 4 days vehicle, P < 0.0001. For HSP70 quantitative analysis, F (3, 6) = 6.709, P = 0.0241. 1 day vehicle vs 0 day, P = 0.0244; 1 day 17-AAG vs 1 day vehicle, P = 0.0279; 4 days 17-AAG vs 4 days vehicle, P = 0.0419Back to article page