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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: HSF1 is involved in suppressing A1 phenotype conversion of astrocytes following spinal cord injury in rats

Fig. 8

Western blot analysis of C3 and HSP70 protein levels in the injured cord. The rats were received 100 μl intraperitoneal injections of 20 mg/kg 17-AAG three times a week on alternate days. a–c Western blot analysis of C3 (a, b) and HSP70 (a, c) protein levels at 0 day, 1 day, 4 days, and 7 days following spinal cord injury. Quantities were normalized to endogenous β-actin. n = 6. Experiments were performed in triplicates. Error bars represent the standard deviation, *P < 0.05, #P < 0.05, two-way ANOVA with Tukey’s test. For C3 quantitative analysis, F (3, 6) = 78.80, P < 0.0001. 1 day vehicle vs 0 day, P < 0.0001; 4 days vehicle vs 0 day, P < 0.0001; 1 day 17-AAG vs 1 day vehicle, P = 0.0028; 4 days 17-AAG vs 4 days vehicle, P < 0.0001. For HSP70 quantitative analysis, F (3, 6) = 6.709, P = 0.0241. 1 day vehicle vs 0 day, P = 0.0244; 1 day 17-AAG vs 1 day vehicle, P = 0.0279; 4 days 17-AAG vs 4 days vehicle, P = 0.0419

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