Fig. 9

Immunostaining of C3-positive astrocytes at lesion sites of the injured cord following treatment with 17-AAG. The rats were received 100 μl intraperitoneal injections of 20 mg/kg 17-AAG three times a week on alternate days. a Immunostaining of C3-positive astrocytes at lesion sites treated with 17-AAG for 0 day, 1 day, 4 days, and 7 days, respectively. b Statistical analysis of (a) in triplicates each 50 fields. Error bars represent the standard deviation, *P < 0.05, ^#P < 0.05, two-way ANOVA with Tukey’s test. F (3, 6) = 12.89, P = 0.0050. 1 day vehicle vs 0 day, P < 0.001; 4 days vehicle vs 0 day, P = 0.0001; 1 day 17-AAG vs 1 day vehicle, P = 0.0072; 4 days 17-AAG vs 4 days vehicle. P = 0.0046. Rectangles indicate the region magnified. Arrows indicate C3⁺S100β⁺ astrocytes. Scale bars, 200 μm or 20 μm in magnification