Fig. 5

Nfe2l2 response in vivo is microglia-dependent. A Schematic representation of the experiment: mice underwent pharmacological depletion of microglia with 100 μg PLX3397 administered every 12 h for 1 week: 20 and 16 h before the end of the experiment mice received two i.p. injection of 75 mg/kg tBHQ. Bioluminescence Imaging (BLI) was acquired from the brain area (see ROI Fig. 1B) or from the dissected brain for each mouse by in vivo (B) and ex vivo imaging (C). Representative acquisitions are shown as radiance photons (p/s/cm²/sr) and represented as pseudocolors, according to the reported scale bar. The measures of bioluminescence signal are reported in the graphs (D) and (E) as fold change (FC) of the radiance photons versus the value obtained before tBHQ administration (day 6) (for the in vivo imaging) (D) or on the value obtained from the vehicle treated mice (for ex vivo imaging) (E). Data are presented as mean ±SD of n=3 independent samples. Differences were not significant, by unpaired t-test