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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: Inhibition of microglial β-glucocerebrosidase hampers the microglia-mediated antioxidant and protective response in neurons

Fig. 8

Mechanism of microglia-to-neuron cross-talk. A Scheme of the transwell (TW) settings used in the experiment reported in B: BV-2 and SK-ARE-luc2 cells were seeded in separate compartment of the TW or in direct contact. B Luciferase enzymatic activity obtained from coculture of SK-ARE-luc2 and BV-2 cells (SK+BV-2), or SK-ARE-luc2 growth alone in the transwell (SK+TW), or growth in separated compartment with microglia (SK+TW+BV-2); luciferase activity is expressed as fold change (FC) versus the RLU value measured for the monocultures of SK-ARE-luc2 (SK) and presented as mean ±SD of n=6 independent samples measured in triplicate. Statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparison test. ***p<0.001. C Schematic representation of the experiment reported in D: SK-ARE-luc2 cells were grown in coculture with BV-2 (SK+BV-2) or in monoculture with the conditioned medium (SK+CM) obtained from the coculture. D Luciferase activity (RLU) is expressed as FC versus the value measured for the SK-ARE-luc2 monocultures (SK) and presented as mean ±SD of n=3 independent samples measured in triplicate. Statistical significance was determined by one-way ANOVA followed by Dunnet’s multiple comparison test versus SK. ***p<0.001. E BV-2 were pre-treated with 25μM nocodazole (noco) or vehicle for 2 h and cocultured SK; luciferase activity is expressed as fold changes (FC) versus the RLU value measured for the vehicle (veh) and presented as violin-plot with media (dashed lines) and quartiles (dotted lines) of n=12 independent samples measured in triplicate. Mean differences are not significant as calculated by unpaired t-test. F BV-2 were pre-treated with 1μM cytochalasin (cyto) or vehicle (veh) for 1 h and cocultured with SK-ARE-luc2; luciferase activity is expressed as fold changes (FC) versus the RLU value measured for the vehicle and presented violin-plot with median (dash line) and quartiles (dot lines) ±SD of n=7 independent samples measured in triplicate

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