Fig. 4

PBA reverses LPS-induced inflammatory effects in RT4 SCs. A Western blot analysis shows the decreased expression levels of TNFα, c-PARP, and TLR4 following LPS and PBA co-treatment for 24 h. B LPS-induced secretion of TNFα was inhibited by PBA treatments. C Western blot depicting the changes in HDAC3 expression levels but not in the levels of the other HDACs after 24 h of treatment with LPS and PBA. D HDAC3 activity induced by 24 h of treatment with LPS was also suppressed after PBA treatment. n = 4. Significance was assessed by one-way ANOVA. Data are presented as the mean ± SEM. *p < 0.05 versus no LPS and no PBA. ^#p < 0.05 versus only LPS. LPS lipopolysaccharide, PBA sodium phenylbutyrate, SCs Schwann cells, TNFα tumor necrosis factor α, c-PARP cleaved poly (ADP) ribose polymerase, TLR4 toll-like receptor 4, HDAC histone deacetylase, ANOVA analysis of variance, SEM standard error of the mean