Fig. 6

PBA administration enhances axonal regeneration and myelination and prevents muscle atrophy after 6 weeks of sciatic nerve transection injury. A Representative images of immunohistochemistry staining for S100β, PGP9.5, and MBP in the middle region of the regenerating nerve from three groups with quantification of positive-stained cells. B Myelin sheath staining using Luxol fast blue in the middle part of the nerve and quantification of positive-stained myelin sheath. C Graph representing the comparison of RGMW between the hydrogel control and PBA groups. D H&E staining of sections of the left gastrocnemius muscle in three groups. n = 4 different rats for each group. Scale bar = 20 μm. Significance in (C) was assessed by Student’s t test. *p < 0.05 versus hydrogel control group. PBA sodium phenylbutyrate, PGP9.5 protein gene product 9.5, MBP myelin basic protein, LFB luxol fast blue, RGMW relative gastrocnemius muscle weight