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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Potentiation of amyloid beta phagocytosis and amelioration of synaptic dysfunction upon FAAH deletion in a mouse model of Alzheimer's disease

Fig. 1

FAAH deletion rescues synaptic plasticity in 5xFAD/FAAH−/− mice. A Input/output curves of fEPSPs evoked at the CA3–CA1 synapse by single pulses of increasing intensities in WT (n = 11), FAAH−/− (n = 13), 5xFAD (n = 10), and 5xFAD/FAAH−/− (n = 12) mice (*p < 0.05 and **p < 0.01, according to the Mann–Whitney test). B Paired-pulse facilitation ratios from synaptic responses evoked by stimulation of the Schaffer collateral fibres with different interstimulus intervals (**p < 0.01 comparing WT/FAAH−/− vs 5xFAD; $p < 0.05 comparing WT/FAAH−/− vs WT; &p < 0.05, &&p < 0.01 comparing WT/FAAH−/− vs 5xFAD/FAAH−/−, according to Mann–Whitney test). C. fEPSPs were recorded from CA3-to-CA1 synapses and normalized to the average baseline value before LTP induction. Left, Time course of fEPSPs before and after theta-burst LTP induction from WT (n = 10), FAAH−/− (n = 13), 5xFAD (n = 10) and 5xFAD/FAAH−/− (n = 12) mice. Right, Average responses collected from the last 10 min of the recording and normalized to the baseline. 5xFAD slices failed to display significant potentiation (p = 0.18, according to Wilcoxon test). On the contrary, WT, FAAH−/−and 5xFAD/FAAH−/− slices were significantly potentiated with respect to their baselines (##p < 0.01, Wilcoxon test). The Mann–Whitney test was used to compare the extent of potentiation among genotypes (*p < 0.05; **p < 0.01). D fEPSPs were recorded from CA3-to-CA1 synapses and normalized to the average baseline value before LTD induction. Left, Time course of fEPSPs before and after induction of NMDA receptor-dependent LTD from WT (n = 8), FAAH−/− (n = 9), 5xFAD (n = 7) and 5xFAD/FAAH−/− (n = 9) mice. Right, Average responses collected from the last 10 min of the recording and normalized to the baseline. WT and FAAH−/− slices were significantly depressed with respect to their baseline, as well as 5xFAD and 5xFAD/FAAH−/− slices (#p < 0.05; ##p < 0.01, according to Wilcoxon test). There were no significant differences among genotypes, according to Mann–Whitney test. For all panels, data are presented as means ± s.e.m. and representative traces are shown on the right

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