Fig. 4

FAAH deletion potentiates activation of microglia and phagocytosis of Aβ in 5xFAD mice. A Expression levels of activated microglia marker CD68 obtained from hippocampal extracts after RT-qPCR. B Representative flow plots of the gating strategy for identification of phagocytic microglia cells (CD11b + CD45 low) isolated from 5 and 5xFAD/FAAH^−/− mice. C Quantification of phagocytic microglial cells (expressed as the percentage of total singlet cells that are CD11 + , CD45lo, Methoxy X04 + cells) in 5xFAD and 5xFAD/FAAH^−/− mice. Statistical analysis: for A, two-way ANOVA followed by Tukey’s test (***p < 0.001, ****p < 0.0001) (n = 7 animals in each group); for C, unpaired t test with Welch’s correction (p = 0.04) (n = 4 animals in each group)