Fig. 7

Only few predicted kinase families drive IFN-α-induced phosphorylation in both microglia and astrocytes. Fold enrichment of motifs extracted from phosphopeptides with increased phosphorylation using motif-X in A microglia and B astrocytes. Kinase families were predicted by matching the enriched motif with their consensus substrate motif and are color coded. C Top kinase families from treated microglia and astrocytes based on substrate motif matches and protein–protein interaction. Percentages of the upregulated phosphopeptides associated with a kinase compared with the total number of phosphopeptides associated with a kinase family per treatment and cell type are shown. D IFN-α-regulated activity score of kinases calculated from the abundance, fold change and role, extracted from PhosphoSitePlus, of each phosphosite on the kinase. Dashed line: threshold score equivalent to a 1.5-fold change in activity