Fig. 6

PKM2 overexpression stimulates phagocytic activity and engulfment of neurons. Transient transfection of BV2 cells with the negative control plasmid (NC) and PKM2 over-expressed plasmid (PKM2); 24 h later, the cells were analyzed for RT-PCR, Western blot, and flow cytometry. A The mRNA expression of PKM2 was detected by RT-PCR. B Western blot quantification of PKM2 on BV2 cells. C Western blot quantification of LAMP1 on BV2 cells. D The expression level of LAMP1 detected by flow cytometry. E After BV2 cells were transfected with NC and PKM2 high expression plasmids, a phagocytic function test was performed, and the phagocytosis of BV2 cells were detected by flow cytometry. F After transfected with NC and PKM2 high expression plasmids, BV2 cells were co-cultured with HT22 cells, then Western blot was used to detect the protein level of PSD95. Data represent the mean scores ± SEM, *P ≤ 0.05, **P ≤ 0.01, ****P ≤ 0.0001. n = 3 per group