Fig. 8

Microglial PKM2 inhibition alleviates brain damage and cognitive disorders in MRL/lpr mice. Twenty female MRL/lpr mice, aged 8 weeks, were divided into two groups: vector group and shPKM2 group. The mice were maintained until week 10. Empty vector virus or AAV9-shPKM2 virus were then stereotactically injected into the lateral ventricle of MRL/lpr mice, until the mice reached week 20. A HE staining of the coronal area of the mouse brain of each group mice; Magnification: Hippocampus (100 ×), CA1 (1000 ×), CA3 (1000 ×), DG (1000 ×), celebrate cortex (1000 ×), n = 4. B Tunel (red) and NeuN (yellow) immunofluorescence double staining of the hippocampus of each group mice; bar = 200 μm, n = 4. C The expression levels of IL-6 of the hippocampus of each group measured by ELISA, n = 6. D The expression levels of IL-1β in the hippocampus of each group measured by ELISA, n = 6. E Western blot quantification of albumin in the hippocampus and cortex, n = 4. F On day 6 of the water maze experiment, the time they stayed in the target quadrant (left) and the number of times the mice crossed the platform (right) was recorded, n = 10. G Fear conditioning tests. The percentage of the stiffness of mice was recorded during the test: different effects of 40-Hz light intervention, 2000-Hz sound intervention, and photoacoustic treatment on contextual and cue memory, respectively, n = 10. Data are represented as mean ± SEM, *P ≤ 0.05, **P ≤ 0.01, ****P ≤ 0.0001