Fig. 9

Silencing microglial PKM2 reduces microglial activation and loss of neuronal synapses by β-catenin signaling pathway. A The percentage of microglia (CD45^loCD11b⁺) in the hippocampus, n = 6. B The expression level of CD86 in microglia detected by flow cytometry, n = 6. C The expression level of LAMP1 in microglia detected by flow cytometry, n = 6. D Western blot quantification of PSD95 and VGLUT1 in the hippocampus of vector and sh-PKM2 group, n = 4. E Western blot quantification of PSD95 and VGLUT1 in the cerebral cortex of vector and sh-PKM2 group, n = 4. F PSD95 (red) and IBA-1 (pink) immunofluorescence double staining in the hippocampus of the mice in each group were detected by IF; Bar = 200 μm, n = 4. G Western blot quantification of β-catenin, c-Myc, and CyclinD1 in the hippocampus of vector and sh-PKM2, n = 4. H Western blot quantification of β-catenin, c-Myc, and CyclinD1 in the cerebral cortex of vector and sh-PKM2, n = 4. Data are represented as mean score ± SEM, *P ≤ 0.05, **P ≤ 0.01, ****P ≤ 0.0001