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Table 1 Antibodies used in the present study

From: Tissue-resident M2 macrophages directly contact primary sensory neurons in the sensory ganglia after nerve injury

 

Catalog no

Host

Clonality

Concentration

Manufacturer

RRID

Primary antibody

 Anti-activating transcription factor 3 (ATF3) (a marker of damaged neuron)

HPA001562

Rabbit

Polyclonal

1:1000

Atlas Antibodies, Stockholm, Sweden

AB_1078233

 Anti-bromodeoxyuridine (BrdU) (a marker of cell-proliferation)

ab6326

Rat

Monoclonal

1:1000

Abcam, Cambridge, UK

AB_305426

 Anti-ionized calcium binding adaptor molecule 1 (Iba1) (a marker of macrophage/microglia)

ab5076

Goat

Polyclonal

1:5000

Abcam, Cambridge, UK

AB_2224402

 Anti-product gene protein 9.5 (PGP9.5) (a marker of neuron)

GP14104

Guinea pig

Polyclonal antiserum

1:5000

Neuromics, Edina, MN

AB_2210625

 Anti-caspase-3 (a marker of cell death)

NB600-1235

Rabbit

Polyclonal

1:500

Novus Biologicals, Littleton, CO

AB_2069897

 Anti-glutamine synthetase (a marker of satellite glial cell)

G2781

Rabbit

Polyclonal

1:10,000

Merck, Darmstadt, Germany

AB_259853

 Anti-mouse cluster of differentiation 206 (CD206) (a marker of M2 phenotype)

AF2535

Goat

Polyclonal

1:1000

R&D Systems, Minneapolis, MN

AB_2063012

 Anti-mouse cluster of differentiation 86 (CD86) (a marker of M1 phenotype)

553,689

Rat

Monoclonal

1:1000

R&D Systems, Minneapolis, MN

AB_394991

Secondary antibody

 Anti-rat IgG-conjugated to Alexa Fluor 488

ab150153

Donkey

Polyclonal

1:800

Abcam, Cambridge, UK

AB_2737355

 Anti-rabbit IgG-conjugated to Alexa Fluor 488

ab150065

Donkey

Polyclonal

1:800

Abcam, Cambridge, UK

AB_2860569

 Anti-rabbit IgG-conjugated to Alexa Fluor 555

ab150066

Donkey

Polyclonal

1:800

Abcam, Cambridge, UK

 

 Anti-rabbit IgG-conjugated to Alexa Fluor 647

ab150067

Donkey

Polyclonal

1:800

Abcam, Cambridge, UK

 

 Anti-goat IgG-conjugated to Alexa Fluor 555

A-21432

Donkey

Polyclonal

1:800

Thermo Fisher Scientific, Waltham, MA

AB_2535853

 Anti-goat IgG-conjugated to Alexa Fluor 647

A-21447

Donkey

Polyclonal

1:800

Thermo Fisher Scientific, Waltham, MA

AB_2535864

 Anti-Guinea pig IgG-conjugated to Alexa Fluor 488

706-545-148

Donkey

Polyclonal

1:800

Jackson ImmunoResearch Labs, West Grove, PA

AB_2340472

 Anti-Guinea pig IgG-conjugated to Alexa Fluor 647

706-605-148

Donkey

Polyclonal

1:800

Jackson ImmunoResearch Labs, West Grove, PA

AB_2340476

 Anti-rabbit IgG-conjugated to biotin

BA-1000

Goat

Polyclonal

1:200

Vector Laboratories, Burlingame, CA

AB_2313606

  1. RRID research resource identifier