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Table 1 Antibodies used in the present study

From: Tissue-resident M2 macrophages directly contact primary sensory neurons in the sensory ganglia after nerve injury

  Catalog no Host Clonality Concentration Manufacturer RRID
Primary antibody
 Anti-activating transcription factor 3 (ATF3) (a marker of damaged neuron) HPA001562 Rabbit Polyclonal 1:1000 Atlas Antibodies, Stockholm, Sweden AB_1078233
 Anti-bromodeoxyuridine (BrdU) (a marker of cell-proliferation) ab6326 Rat Monoclonal 1:1000 Abcam, Cambridge, UK AB_305426
 Anti-ionized calcium binding adaptor molecule 1 (Iba1) (a marker of macrophage/microglia) ab5076 Goat Polyclonal 1:5000 Abcam, Cambridge, UK AB_2224402
 Anti-product gene protein 9.5 (PGP9.5) (a marker of neuron) GP14104 Guinea pig Polyclonal antiserum 1:5000 Neuromics, Edina, MN AB_2210625
 Anti-caspase-3 (a marker of cell death) NB600-1235 Rabbit Polyclonal 1:500 Novus Biologicals, Littleton, CO AB_2069897
 Anti-glutamine synthetase (a marker of satellite glial cell) G2781 Rabbit Polyclonal 1:10,000 Merck, Darmstadt, Germany AB_259853
 Anti-mouse cluster of differentiation 206 (CD206) (a marker of M2 phenotype) AF2535 Goat Polyclonal 1:1000 R&D Systems, Minneapolis, MN AB_2063012
 Anti-mouse cluster of differentiation 86 (CD86) (a marker of M1 phenotype) 553,689 Rat Monoclonal 1:1000 R&D Systems, Minneapolis, MN AB_394991
Secondary antibody
 Anti-rat IgG-conjugated to Alexa Fluor 488 ab150153 Donkey Polyclonal 1:800 Abcam, Cambridge, UK AB_2737355
 Anti-rabbit IgG-conjugated to Alexa Fluor 488 ab150065 Donkey Polyclonal 1:800 Abcam, Cambridge, UK AB_2860569
 Anti-rabbit IgG-conjugated to Alexa Fluor 555 ab150066 Donkey Polyclonal 1:800 Abcam, Cambridge, UK  
 Anti-rabbit IgG-conjugated to Alexa Fluor 647 ab150067 Donkey Polyclonal 1:800 Abcam, Cambridge, UK  
 Anti-goat IgG-conjugated to Alexa Fluor 555 A-21432 Donkey Polyclonal 1:800 Thermo Fisher Scientific, Waltham, MA AB_2535853
 Anti-goat IgG-conjugated to Alexa Fluor 647 A-21447 Donkey Polyclonal 1:800 Thermo Fisher Scientific, Waltham, MA AB_2535864
 Anti-Guinea pig IgG-conjugated to Alexa Fluor 488 706-545-148 Donkey Polyclonal 1:800 Jackson ImmunoResearch Labs, West Grove, PA AB_2340472
 Anti-Guinea pig IgG-conjugated to Alexa Fluor 647 706-605-148 Donkey Polyclonal 1:800 Jackson ImmunoResearch Labs, West Grove, PA AB_2340476
 Anti-rabbit IgG-conjugated to biotin BA-1000 Goat Polyclonal 1:200 Vector Laboratories, Burlingame, CA AB_2313606
  1. RRID research resource identifier