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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Tracking distinct microglia subpopulations with photoconvertible Dendra2 in vivo

Fig. 3

Quantification of photoconverted D2 cells using flow cytometry. A In vivo SLO imaging of a Cx3cr1+/GFP retina, a naive Cx3cr1+/D2 retina (unconverted), and a photoconverted Cx3cr1+/D2 retina that was exposed to 405 nm light across the entire retinal surface (110 µW, 780 × 780 µm per quadrant, 3.5–5 J/cm2 radiant exposure). Scale 300 µm. B Flowcytometry gates used to identify single cells, alive, double positive for Cd11b+ CD45+, and with high expression of Cx3cr1, as measured by green fluorescence to exclude peripheral monocytes. C Quantification of green gD2 cells and green and red double positive rD2 cells from each condition. The Cx3cr1+/GFP retina was composed entirely of green, GFP+ microglia, similar to the Cx3cr1+/D2 unconverted retina which was nearly all green gD2 microglia, with a negligible population of rD2 fluorescence. Conversely, the Cx3cr1+/D2 photoconverted retina had fewer gD2 cells and a large population of rD2 cells. Error bars are SE, n = 3 retinas per group (5 mice), all retinas were from male mice

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