Fig. 4

Acute cerebral I/R injury increases the non-nuclear abundance of neuronal-derived rRNA and promotes pro-inflammatory cytokine expression. a Mice underwent 60 min MCAO followed by 2 h or 4 h of reperfusion or were subjected to sham surgery. Co-immunofluorescent staining was performed to determine the nuclear, peri-nuclear and non-nuclear abundance of neuronal rRNA across the striatum and cortex of the contra- and ipsilateral brain hemisphere (n = 3–4 per group; One-way ANOVA with Holm–Sidak's multiple comparisons test; * p < 0.05, ** p < 0.01, *** p < 0.001). b Mice underwent 60 min MCAO followed by 12 h reperfusion or were subjected to sham surgery. Real-time RT-PCR was used to determine transcript levels of pro-inflammatory cytokines. Values are normalized to Rps12 (n = 3 per group; One-way ANOVA with Holm–Sidak's multiple comparisons test; * p < 0.05, ** p < 0.01, *** p < 0.001). c Representative microphotographs of rRNA and NeuN immunofluorescence stainings in brain tissue cyrosections of mice subjected either to ischemic stroke or sham surgery: rRNA (red), NeuN (green) and nuclei (blue). Scale bars = 30 µm and 15 µm (magnified sections)