Fig. 10

Flow cytometry analysis was used to identify proportions of myeloid cells at P120 and reveals distinct changes in T cells in CHORIO rats. Notably, proportions of viable (identified by size, granularity and viability dye staining, A–D) T helper cells (CD45⁺CD3⁺CD4⁺) in the brain (G), and T regulatory cells (CD45⁺CD3⁺ CD4⁺CD25⁺) in the brain (H) and blood (J) are increased in CHORIO rats compared to sham controls. Similarly, the proportion of cells with positive expression for infiltrating macrophage markers (CD45^highCD11b⁺) are also increased in the brain of CHORIO rats at P120 compared to sham (I). Gating for CD3⁺ and CD25⁺ cells is shown in E and F (^*p < 0.05, FSC  forward scatter, SSC  side scatter). (Data represent at least 3 independent experiments.)