Fig. 2

RhoA cKO in macrophages inhibits the degeneration of axons and myelin in the injured nerve. a–d Western blots and statistical analysis illustrating that the levels of NF200 and P0 protein at 3dpi (a, b) and 7dpi (b, c) are significantly higher in cKO group than those of the Cre group. e Immunohistochemistry in the longitudinal sections of transected sciatic nerve’s distal trunk, which showing the axons (NF200⁺) and myelin sheaths (P0⁺) are fragmented and get worse from 3 to 7 dpi. f–k Quantification of the length distributions of axons (f, g) and myelin fragments (h, i) as well as the NF200 (j) and P0 (k) immunointensities showing both the length of axonal fragments and myelin fragments are significantly higher in the cKO group than those of the Cre group at both of 3 dpi and 7 dpi. Scale bars: 50 µm. Data are expressed as the mean ± SEM (n = 6 per group)