Fig. 5

RhoA cKO inhibits the migration of macrophages in vivo and in vitro. a F4/80 immunohistochemistry in the cross sections of crush injured sciatic nerve’s distal trunk at 0, 3, 7, 14, 21, 28 dpi. Scale bars: 50 µm. b Quantification of the number of macrophages in each time point. c, d Transwell assay and quantitative analysis showing the number of migrated macrophages in cKO group is significantly less than Cre group in vitro. Scale bars: 100 µm. Data are expressed as the mean ± SEM (n = 6 per group)