Fig. 6

RhoA cKO inhibits the phagocytosis of macrophages in vivo and in vitro. a–c F4/80 and ORO double staining in the crush injured sciatic nerve at 7 dpi showing that the ratio of ORO⁺ macrophages and the ORO⁺ area in each macrophage are both significantly decreased in the cKO group comparing to the Cre group. Scale bars: 50 µm. d, e F4/80 immunocytochemistry with the fluorescent lumispheres and quantitative analysis of the number of lumispheres per macrophage in vitro. Scale bars: 50 µm. f–k F4/80 and ORO f–h/MBP i–k double immunocytochemistry staining and quantitative analysis after the macrophages cultured with myelin debris in vitro. Scale bars: 50 µm. l–m Western blots and quantification showing that MBP expression in cKO group cultured with myelin debris is less than those in Cre group in vitro. Data are expressed as the mean ± SEM (n = 6 per group)