Fig. 8

Y27632 and ML7 treatment inhibit macrophage migration and phagocytosis in vitro. a, b Transwell assay and quantitative analysis showing the number of migrated macrophages from Y27632 group and ML7 group is significantly less than the control (CTRL) group. Scale bars: 100 µm. c, d F4/80 immunocytochemistry with the fluorescent lumispheres and quantitative analysis. Scale bars: 50 µm. e–j F4/80 and ORO. e–g /MBP. h–j Double immunocytochemistry staining and quantification of the ratio of ORO⁺ macrophages and the ORO⁺ area in each cell after the macrophages cultured with myelin debris in vitro. Scale bars: 50 µm. k–l Western blots and quantification showing that MBP expression in Y27632 group and ML7 group cultured with myelin debris are less than those in CTRL group in vitro. Data are expressed as the mean ± SEM (n = 6 per group)