Fig. 8From: Prostaglandin EP2 receptor antagonist ameliorates neuroinflammation in a two-hit mouse model of Alzheimer’s diseaseEP2 antagonist treatment increases amyloid deposition in two-hit 5xFAD female brain. Different brain regions of the 5xFAD females from two-hit and single-hit—environmental cohorts treated either with vehicle or TG11-77.HCl are stained with Congo red. Microscopic images reveal numbers and size of amyloid plaques in vehicle (A–C) and TG11-77.HCl treated mice (D–F). ImageJ quantification demonstrates number, size and % area covered by the plaques in different regions of the brain after TG11-77.HCl treatment in two-hit (G–I) and single-hit genetic mice (J–L). Paired t test was applied between groups and multiple unpaired t test with FDR (5%) was applied for multiple comparison. P values were set to be significant at * ≤ 0.05. Scale bar = 50 mm. Data are mean ± SEM. CCx cerebral cortex, ECx entorhinal cortex, PCx piriform cortex, Hippo hippocampus, CC corpus callosumBack to article page