Table 1 Mouse primer sequences used in qRT-PCR reactions
Genes | Forward Primer (sequence 5′–3′) | Reverse Primer (sequence 5′–3′) |
|---|---|---|
βActin | AAGGCCAACCGTGAAAAGAT | GTGGTACGACCAGAGGCATAC |
GAPDH | TGTCCGTCGTGGATCTGAC | CCTGCTTCACCACCTTCTTG |
HPRT1 | GGAGCGGTAGCACCTCCT | CTGGTTCATCATCGCTAATCAC |
IL-6 | TCTAATTCATATCTTCAACCAAGAGG | TGGTCCTTAGCCACTCCTTC |
CCL2 | CATCCACGTGTTGGCTCA | GCTGCTGGTGATCCTCTTGTA |
TNF | TCTTCTGTCTACTGAACTTCGG | AAGATGATCTGAGTGTGAGGG |
IL1β | TGAGCACCTTCTTTTCCTTCA | TTGTCTAATGGGAACGTCACAC |
EP2 | TCTTTAGTCTGGCCACGATGCTCA | GCAGGGAACAGAAGAGCAAGGAGG |
Iba1 | GGATTTGCAGGGAGGAAAAG | TGGGATCATCGAGGAATTG |
GFAP | GACAACTTTGCACAGGACCTC | ATACGCAGCCAGGTTGTTCT |
CD11b | CCAGTAAGGTCATACAGCATCAGT | TTGATCTGAACAGGGATCCAG |
S100B | TCGGACACTGAAGCCAGAG | AGACATCAATGAGGGCAACC |