Fig. 4

MiR-204-5p knock-down within the hippocampal DG region results in neuronal inflammation and oxidative stress in normal rats. A Representative confocal microscopic images showing expressions of 4-HNE in the DG region after AAV-miR-204-5p sponge infusion (N = 6 per group). Scale bar is 50 μm. B Representative images of Mito-Sox staining (red). Nuclei (blue) are stained with DAPI. Neurons (green) are stained with Anti-NeuN. Scale bar is 50 μm. (N = 6 per group). C Representative images of 8-oHdG staining (red). Scale bar is 50 μm. (N = 6 per group) D Immunofluorescent staining of Iba1 positive microglial cells within the DG region. Scale bar is 50 μm. (N = 6 per group) E Western bolt assays of protein expression levels of NOX1 and NOX4 within the DG region (N = 6 per group). F Q-PCR assays of mRNA expression levels of IL1β, TNF-α and IFN-γ within the DG region. Band intensities were normalized to GAPDH (N = 6 per group). **P < 0.01 ***P < 0.001, ****P < 0.0001, control + AAV-control vs control + AAV-miR-204-5p sponge. Data are presented as means ± SEMs. Student t-tests were employed for comparisons between the two groups