Fig. 8
From: Interleukin-6 mediates delirium-like phenotypes in a murine model of urinary tract infection
Systemic IL-6 inhibition fully reverses UTI-induced neuronal changes to the frontal cortex and hippocampus. a, b Quantification of cleaved caspase-3 (CC3) in the frontal cortex (ANOVA, F = 24.01, dF = 54, p < 0.0001) and the hippocampus (ANOVA, F = 34.92, dF = 54, p < 0.0001) showed significant reduction of CC3 expression in the anti-IL-6 antibody treated group compared to the UTI group. Each dot represents one animal (n = 16 in control, n = 20 in UTI, n = 19 in UTI + α-IL-6). c Representative sections stained for CC3 (positive signal displayed in green) with both the neuronal marker, NeuN (red), and DAPI nuclear stain (blue). Magnified regions, indicated by dashed lines, of an area including both the frontal neocortex and hippocampus from the micrograph directly above reveal CC3 expression within neurons. Quantitative data are expressed in mean ± SD, ***p < 0.001, ****p < 0.0001