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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: The balance between AIM2-associated inflammation and autophagy: the role of CHMP2A in brain injury after cardiac arrest

Fig. 1

Expression levels of inflammasome components in the cortex after CA-ROSC. a Transcript levels of the inflammasome sensor genes AIM2, NLRP3, NLRP1, and NLRC4 in cortical tissues from sham and CA-ROSC animals. n = 4 per group. The data are presented as means ± SEM, Student’s t test. *P < 0.05 and ****P < 0.0001 vs. sham. b, c Representative Western blot and quantification of AIM2 in cortical tissue lysates from sham rats and at the indicated timepoints (1.5, 3, 6, 12, and 24 h) after CA. d, e Representative Western blot and quantification of total caspase-1, ASC, IL-1β, and IL-18 protein levels in cortical tissue lysates of sham rats and at the indicated timepoints (1.5, 3, 6, 12, and 24 h) after CA. f Colocalization of AIM2 (red) with neurons (NeuN, green), microglia (Iba-1, green), and astrocytes (GFAP, green) in the cortex 12 h after CA. Scale bar, 50 μm. g, h Representative immunofluorescence images and quantification of AIM2 and NeuN double staining. i, j Representative immunofluorescence images and quantification of caspase-1 and NeuN double staining. Scale bar, 50 μm. The data are expressed as means ± SEM (n = 5), one-way ANOVA. **P < 0.01, ***P < 0.001, and ****P < 0.0001 vs. sham

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