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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: The balance between AIM2-associated inflammation and autophagy: the role of CHMP2A in brain injury after cardiac arrest

Fig. 2

Modulating autophagy affects the AIM2 inflammasome. a, b Representative Western blot and quantification of GSDMD in the cortical tissue lysates of sham and CA-ROSC rats at the indicated timepoints. c, d Representative immunofluorescence images and quantification of GSDMD and NeuN double-staining. Scale bar, 50 μm. e Western blot analysis of LC3, p62, AIM2, IL-1β, and IL-18 in PC12 cells stimulated with or without LPS (500 ng/mL) for 8 h and then treated with Rapa (5 μmol/L), CQ (10 μmol/L), Rapa (5 μmol/L) plus CQ (10 μmol/L), or vehicle control for 24 h. f Quantification of LC3, p62, AIM2, IL-1β, and IL-18 in PC12 cell lysates. The data are expressed as means ± SEM (n = 3–6), one-way ANOVA. **P < 0.01, ***P < 0.001, and ****P < 0.0001

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