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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: The balance between AIM2-associated inflammation and autophagy: the role of CHMP2A in brain injury after cardiac arrest

Fig. 3

Impaired autophagic flux and expression levels of ESCRT-III components in cortical neurons after CA. a Representative Western blot showing Beclin-1, SQSTM1/p62 and LC3 in the cortical tissue lysates of sham and CA-ROSC rats at the indicated timepoints. b Quantification of Beclin-1, SQSTM1/p62 and LC3 with respect to the loading control β-actin. c Representative Western blot showing CHMP2A, CHMP2B, CHMP3, CEP55 and CHMP7 in the cortical tissue lysates of sham and CA-ROSC rats at the indicated timepoints. d Quantification of ESCRT-III components with respect to the loading control β-actin. e, f Representative immunofluorescence images of SQSTM1/p62 and LC3 double-staining and the quantification of LC3-positive cells (yellow bars) and LC3 and SQSTM1/p62 double-positive cells (red bars) in the cortex of sham-operated and injured rats. g, h Representative immunofluorescence images and the quantification of CHMP2A and NeuN double-staining. Scale bar, 50 μm. i, j Representative immunofluorescence images and quantification of LC3 and NeuN double staining. k, l Representative immunofluorescence images and quantification of SQSTM1/p62 and NeuN double staining. Scale bar, 50 μm. The data are expressed as means ± SEM (n = 4–6), one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 vs. sham

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