Fig. 6

AIM2 knockdown abolishes OGD-induced autophagy damage and inflammation activation. a, b Representative Western blot and quantification of autophagy-related factors (LC3, p62) and AIM2-related factors (AIM2, total caspase-1, ASC, IL-1β, and IL-18) in PC12 cells infected with AIM2-specific shRNA (MOI = 20) or vector shRNA (MOI = 20) for 72 h and then subjected to OGD-Rep or normoxia (Nor) as a control. (n = 6, one-way ANOVA. **P < 0.01 and ****P < 0.0001). c Schematic showing that the Lenti-EGFP vector or Lenti-EGFP-CHMP2A virus was injected into the motor cortex. d Double immunofluorescence images of green fluorescence and CHMP2A protein (red) in the motor cortex on day 7 after Lenti-EGFP-CHMP2A virus injection. Scale bar, 1000 μm. e, f CHMP2A protein levels were elevated by LV-CHMP2A. (n = 5, Student’s t test. ****P < 0.0001 vs. LV-Vector group)