Fig. 2
From: CD4+ effector T cells accelerate Alzheimer’s disease in mice
Antigen-specificity of the Aβ-Th1 and Aβ-Th17 cells a MHCII-IAb–KLVFFAEDVGSNKGA (Aβ T cell epitope) tetramer binding with Aβ-Th1 and Aβ-Th17 cells after incubation. Control tetramer MHCII-IAb–PVSKMRMATPLLMQA was used for precise gating of Aβ T cell epitope recognizing CD4+ T cell population. b From Aβ-Th1 cells, antigen-recognizing variable regions of T cell receptor (TCR) alpha (α) and beta (β) chains were identified using molecular cloning. Molecular modeling of full-length TCRα/β complex with Aβ1–42–MHCII-IAb (pMHC) complex; MHCII-IAbα chain (green), MHCII-IAbβ chain (cyan) and peptide (blue); TCRα chain (yellow) and TCRβ chain (red). The interface of MHCII, peptide and TCR binding is shown by encircled region. c (i) peptide surface at the interface of MHC and TCR. Peptide–MHC interactions; (ii) peptide Ala563 and Arg322 interaction with MHCα Arg168 and MHCβ Arg322, respectively; (iii) peptide Lys549 interaction with MHCα Arg168(O); (iv) peptide Lys549 interaction with MHCα Asp169(OD2); TCR–pMHC interactions- (v) pMHC Glu543 and Ser547 interaction with Tyr908, Asp953 and Gln952. (vi) pMHC His535 (NE2) interaction with Asn(O), and His535 (O) interaction with Tyr956 (OH), π–π interactions between Tyr956 and His535; (vii) pMHC interaction with TCRβ, Glu532(OE2)–Tyr961(OH) and Glu532 (OE1)–His886(NE2)