Fig. 6
From: CD4+ effector T cells accelerate Alzheimer’s disease in mice
Aβ-Th1 and Aβ-Th17 cells increase amyloid load in APP/PS1 mice. a Western blot analysis performed to determine expression of full-length APP using cortical tissue lysate and 22C11 and 6E10 antibodies. Representative immunoblot and densitometric quantification was performed using different APP/PS1 mice. n = 4 mice per group were analyzed for statistical significance using one-way ANOVA followed by Newman–Keuls post hoc test. b ELISA performed to quantify Aβ1–40 and Aβ1–42 levels in the brain using Tris–HCl soluble fractions of cortical tissue. c Immunohistochemistry (pan-Aβ) and immunofluorescence (Thioflavin-S) performed to determine the area occupied by the insoluble Aβ plaque in the cortex and hippocampal brain regions. Representative images showing amyloid plaque DAB and Thioflavin-S staining in different brain regions. Percentage occupied area quantified using Cavalieri estimator probe of Stereo Investigator system (MBF Bioscience). Scale bar = 100 µm. n = 6 mice per group were analyzed. Statistical differences between groups determined using one-way ANOVA followed by Newman–Keuls post hoc test. *p < 0.05, **p < 0.01