Fig. 3From: The effect of macrophage polarization on the expression of the oxytocin signalling system in enteric neuronsEffect of cytokines in macrophages supernatant on the expression of OT and OTR in enteric neurons. A–C M1 supernatant treatment for 24 h increased IL-6, TNF-α and IL-1β levels in cultured enteric neurons as assessed by ELISA. D M2 supernatant treatment for 24 h increased TGF-β levels in cultured enteric neurons as assessed by ELISA. E–G Stimulation of cultured enteric neurons for 6 h with IL-6 (0, 5, 10, 20 ng/ml), TNF-α (0, 10, 20, 40 ng/ml) or IL-1β (0, 0.1, 1, 10 ng/ml) decreased the mRNA expression of OT and OTR. H Stimulation of cultured enteric neurons with IL10 (0, 2, 10, 20 ng/ml) did not affect the expression of OT and OTR mRNA. I Stimulation of cultured enteric neurons with TGF-β (0, 5, 10, 20 ng/ml) for 6 h increased the mRNA expression of OT and OTR. J The concentration of OT was measured in cultured enteric neurons treated with or without IL-6 (10 ng/ml), TNF-α (20 ng/ml), and IL-1β (1 ng/ml) for 30 min by ELISA. The values represent the mean ± SEM of 6 samples and were compared by one-way ANOVA with Newman–Keuls and Dunnett’s test for multiple comparisons. *p < 0.05, **p < 0.01, ***p < 0.001Back to article page