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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: The effect of macrophage polarization on the expression of the oxytocin signalling system in enteric neurons

Fig. 3

Effect of cytokines in macrophages supernatant on the expression of OT and OTR in enteric neurons. A–C M1 supernatant treatment for 24 h increased IL-6, TNF-α and IL-1β levels in cultured enteric neurons as assessed by ELISA. D M2 supernatant treatment for 24 h increased TGF-β levels in cultured enteric neurons as assessed by ELISA. E–G Stimulation of cultured enteric neurons for 6 h with IL-6 (0, 5, 10, 20 ng/ml), TNF-α (0, 10, 20, 40 ng/ml) or IL-1β (0, 0.1, 1, 10 ng/ml) decreased the mRNA expression of OT and OTR. H Stimulation of cultured enteric neurons with IL10 (0, 2, 10, 20 ng/ml) did not affect the expression of OT and OTR mRNA. I Stimulation of cultured enteric neurons with TGF-β (0, 5, 10, 20 ng/ml) for 6 h increased the mRNA expression of OT and OTR. J The concentration of OT was measured in cultured enteric neurons treated with or without IL-6 (10 ng/ml), TNF-α (20 ng/ml), and IL-1β (1 ng/ml) for 30 min by ELISA. The values represent the mean ± SEM of 6 samples and were compared by one-way ANOVA with Newman–Keuls and Dunnett’s test for multiple comparisons. *p < 0.05, **p < 0.01, ***p < 0.001

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