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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: The effect of macrophage polarization on the expression of the oxytocin signalling system in enteric neurons

Fig. 4

Effects of cytokine receptor antagonistson OT and OTR expression in cultured enteric neurons. A Cultured enteric neurons were pretreated with tocilizumab (IL-6-Ra, 1 μM) for 12 h followed by IL-6 (10 ng/ml) for 6 h. The relative mRNA expression levels of OT and OTR were analysed by qRT-PCR. B Cultured enteric neurons were pretreated with R-7050 (TNF-α receptor antagonist, 1 μM) for 12 h followed by TNF-α (20 ng/ml) for 6 h. The relative mRNA expression levels of OT and OTR were analysed by qRT-PCR. C Cultured enteric neurons were pretreated with LY2109761 (TGF-β receptor antagonist, 1 μM) for 12 h followed by TGF-β (10 ng/ml) for 6 h. The relative mRNA expression levels of OT and OTR were analysed by qRT-PCR. D Cultured enteric neurons were pretreated with tocilizumab, R-7050 or IL-1ra for 12 h followed by M0 and M1 supernatant for 24 h. The relative expression levels of OT and OTR mRNA were analysed by qRT-PCR. Notably, the three receptor antagonists reversed the effect of M1 supernatant on the expression of OT. E Cultured enteric neurons were pretreated with LY2109761 for 12 h followed by M0 or M2 supernatant for 24 h. The relative expression levels of OT and OTR mRNA were analysed by qRT-PCR. LY2109761 downregulated the mRNA expression of OT and OTR by inhibiting the expression of TGF-β. Values represent the mean ± SEM of 6 samples and were compared by one-way ANOVA with Newman–Keuls and Dunnett’s test for multiple comparisons. *p < 0.05, **p < 0.01, ***p < 0.001

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