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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: The effect of macrophage polarization on the expression of the oxytocin signalling system in enteric neurons

Fig. 7

Correlation between macrophage polarization and the expression of OT and OTR in DSS-induced colitis. A–D The mRNA levels of TNF-α, IL-6, IL-1β, CCL2, and iNOS in the LMMP were measured in different periods of the DSS model by qRT-PCR. In the first and second weeks, the mRNA levels of IL-1β, IL-6, TNF-α, CCL2, and iNOS were upregulated. The above results suggested that M1 macrophages polarization. E–G The mRNA levels of TGF-β, CD206, ChIL3, and Arg1 in the LMMP were measured in different periods of the DSS model by qRT-PCR. From the third week of the model, the levels of TGF-β, CD206, ChIL3, and Arg1 mRNA were upregulated. These results suggested M2 macrophage polarization. H, I The levels of IL-6, TNF-α, IL-1β and TGF-β proteins were measured in LMMP by ELISA. J OT and OTR mRNA levels in LMMP were measured in different periods of the DSS model by qRT-PCR. The expression of OT and OTR decreased as inflammation occurred and increased as inflammation subsided. K The level of OT protein in colon explants was measured in different periods of the DSS model by ELISA. The values represent the mean ± SEM of 7 samples and were compared by one-way ANOVA with Dunnett’s test for multiple comparisons. *p < 0.05, **p < 0.01, ***p < 0.001

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