Fig. 2
From: Hypothermia modulates myeloid cell polarization in neonatal hypoxic–ischemic brain injury
Hypothermia protects from delayed neuronal cell death. Nine-day-old C57BL/6 mice were exposed to HI followed by 4 h hypothermia or normothermia. Animals were sacrificed and analyzed via immunohistochemistry 1, 3 or 7 days after HI. Cellular degeneration (a) and neuronal loss (b) were assessed by quanfication of TUNEL+ cells and NeuN+ areas, respectively, in the cortex (Ctx), hippocampus (Hc) and thalamus (Thal). Representative images in a are derived from the CA1 (NeuN) and CA2 (TUNEL) regions of the hippocampus, scale bar: 20 µm. **p < 0.01, ***p < 0.001. n = 7/treatment and timepoint