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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Hypothermia modulates myeloid cell polarization in neonatal hypoxic–ischemic brain injury

Fig. 4

Hypothermia alters temporal expression of typical M1 and M2 marker proteins in the hippocampus. Nine-day-old C57BL/6 mice were exposed to HI followed by 4 h hypothermia or normothermia. Animals were sacrificed and analyzed via immunohistochemistry 1, 3 or 7 days after HI. Myeloid cell polarization was assessed via immunohistochemistry for the prototypical M1 marker CD86 (green) and the M2 marker CD206 (red) in the hippocampus (a, representative images are derived from the CA2 region of the hippocampus from an NT-treated animal 3 days after HI. Asterisks, paragraph symbols and rhombi indicate CD86 single, CD206 single and CD86/CD206 double positive cells, respectively; scale bar: 50 µm). CD86 single (b), CD206 single (c) and CD86/CD206 double (d) positive cells were quantified. Dashed lines and grey shadows in the graphs indicate quantiles (i.e., median and 25th, 75th percentiles) of sham-operated animals across all timepoints, statistical comparisons to sham groups were performed for animals of the same timepoint. Detailed time course analyses of sham-operated animals are provided in the Additional file 1: Fig. S4a. *p < 0.05; # p < 0.05, ## p < 0.01 vs. sham. n = 5 (sham)/timepoint, n = 7 (HI)/treatment and timepoint

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