Fig. 7
From: Hypothermia modulates myeloid cell polarization in neonatal hypoxic–ischemic brain injury
Hypothermia suppresses HI-induced upregulation of a large number of inflammatory genes in myeloid cells. To investigate a large set of genes associated with different myeloid cell polarization states, mRNA expression analyses was performed via real time PCR on MACS-sorted CD11b+ cells from brains of C57Bl/6 mice exposed to HI at P9 followed by immediate HT or NT. Uninjured sham-operated animals served as controls. A broad set of typical M1 (red heading), immunoregulatory (orange heading) and M2 (green heading) marker genes were analyzed. Beta-2-microglobulin served as housekeeping gene and fold change values compared to sham-operated control animals were calculated. *p < 0.05, **p < 0.01, ***p < 0.001, n = 6 animals/timepoint for sham (left and right hemisphere pooled), n = 12 animals/timepoint for HI + NT and for HI + HT (2 ipsilateral hemispheres pooled per sample)