Fig. 7

TRPV4 activation enhances TNF-α production in cultured Müller cells. A Morphology of cultured Müller cells. Scale bar: 50 µm. B GSK101 treatment enhanced GFAP protein levels in cultured Müller cells. n = 4. **p < 0.01 and ***p < 0.001. C Cumulative changes in GFAP protein levels in control and GSK101 treatment groups. D–F Cumulative changes in mRNA levels of TNF-α (D), IL-1β (E), and IL-6 (F) in Müller cell extracts obtained after saline treatment (control) or GSK101 treatment for 24 h. Relative abundances of mRNA were quantified using the 2^−ΔΔct calculation method and are expressed as fold changes. n = 3 for all groups. *p < 0.05 vs control. G, GSK101 treatment led to enhancement of TNF-α protein level. H Cumulative changes in TNF-α protein levels in control and GSK101 treatment groups. n = 6. *p < 0.05 and ***p < 0.001