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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Metabolic reprogramming mediates hippocampal microglial M1 polarization in response to surgical trauma causing perioperative neurocognitive disorders

Fig. 5

Surgical trauma-induced microglial M1 polarization and neuroinflammation in hippocampus were attenuated by 2-DG via glycolytic inhibition in aged mice. A The lactate/pyruvate ratio (L/P ratio) in hippocampus was examined by a Colorimetric Assay Kit; B Microglia were separated by Percoll gradient centrifugation for L/P ratio measurement and flow cytometric analysis; C The lactate/pyruvate ratio (L/P ratio) in enriched microglia was examined by a Colorimetric Assay Kit; D Quantification of the percentage of M1(CD86+CD206−) and M2(CD86−CD206+) phenotype in CD45+CD11b+ microglia enriched by Percoll gradient separation at 3 d after the surgery; E Representative dot plots showing the microglial phenotype of either M1 or M2 polarization examined by flow cytometry; F Western blotting images representing the expression of IL-1β and IL-6 in hippocampus; G Quantification for the protein levels of IL-1β and IL-6 in hippocampus at 3 d after the surgery. *P < 0.05, **P < 0.01 versus control group; #P < 0.05, ##P < 0.01 versus surgery group by one-way ANOVA followed by Tukey post hoc test. n = 5. Data are represented as mean ± SD

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