Fig. 4
From: Administration of anti-ERMAP antibody ameliorates Alzheimer’s disease in mice
Anti-ERMAP mAb does not alter the expression of APP expression and APP-processing enzymes. 3XTg-AD mice were injected with anti-ERMAP mAb, or control isotype Ab as in Fig. 3. Three and a half months later, the brains were harvested. A, B The expression levels of APP and PS-1 proteins as well as the APP cleavage products β-CTF and α-CTF were analyzed by Western blot. GAPDH was used as a loading control. A Representative Western blot images, B quantification of the expression levels of APP and PS-1 proteins. C qRT-PCR analysis of the relative expression levels of the genes for the APP-processing enzymes ADAM10, ADAM17, BACE1, and BACE2. D, E Western blot analysis of the expression levels of ADAM10, ADAM17 and BACE1 proteins. B, C, E The expression level of the protein or gene in control Ab-treated AD mice is defined as 1. The data are expressed as mean ± SD from one of three independent experiments with similar results (5 to 8 mice per group in each experiment)