Fig. 3
The decrease of both TRPC4 and TRPC5 in the hippocampi of Cx3cr1CreER IL-10−/−mice. a RT-PCR results of TRPC isoforms in the hippocampus (n = 3 in each group, t-test). The marked significance is the comparison of the same isoform in the Cx3cr1CreER IL-10−/− with the littermate. b western blotting bands of TRPC1, TRPC3, TRPC4, and TRPC5 in littermates and in Cx3cr1CreER IL-10−/−mice. Bar graphs represent densitometric plots of protein expression in littermate and Cx3cr1CreER IL-10−/−mice in TRPC1 (c), TRPC3 (d), TRPC4 (e), and TRPC5 (f). Each dot represents a mouse. Bars represent mean ± SEM. n = 4 in each group, Significant differences were established by t-test. g Expression of TRPC1, TRPC3, TRPC4, and TRPC5 in the CA3 region of mouse hippocampal slices. Immunofluorescence images were captured with a 20 × objective. Green, immunoreactivity of TRPC1, TRPC3, TRPC4, and TRPC5; blue, nuclei stained with DAPI. Merged images of each TRPC isoform and DAPI staining. *P < 0.05, **P < 0.01, ***P < 0.001