Fig. 5
Neuroinflammatory activity enhanced in the hippocampus in Cx3cr1CreERIL-10−/−mice. a Western blotting bands of inflammation-related molecules in littermates and Cx3cr1CreER IL-10−/−mice. Bar graphs represent densitometric plots of protein expression in littermate and Cx3cr1CreERIL-10−/− mice in GFAP (b), Iba1 (c), NLRP3 (d), caspase-1 (e), Pro-IL-1β (f), IL-1β (g), Pro-IL-18 (h), IL-18 (i), ASC (j), IL-10 (k), and IL-6 (l). Each dot represents a mouse. Bars represent mean ± SEM. n = 4 in each group. Significant differences were established by t-test. m Expression of GFAP and Iba1 in CA3 region in mice hippocampal slices. Immunofluorescence images, captured with a 10 × objective. Green, immunoreactivity of GFAP and Iba1; blue, nuclei staining with DAPI. The merged images of GFAP, Iba1 and DAPI staining. n Immunofluorescence co-localization of NLRP3 and Iba1 in CA3 region in mice hippocampal slices. Immunofluorescence images, captured with a 10 × objective. Green, immunoreactivity of NLRP3; Red, immunoreactivity of Iba1; Blue, nuclei staining with DAPI. The merged images of NLRP3, Iba1 and DAPI staining. *P < 0.05, **P < 0.01