Fig. 8

Upregulation of TRPC5 in hippocampal excitatory neurons inhibiting the neuroinflammation in hippocampus. a–c Expression of GFAP (a), Iba1 (b), and NLRP3 (c) in the CA3 region of hippocampal slices. Immunofluorescence images were captured with a 10 × objective. Red, immunoreactivity of GFAP, Iba1, and NLRP3; blue, nuclei stained with DAPI. The merged images of GFAP, Iba1 and DAPI staining. Bars represent positive cells in GFAP (d), Iba1 (e) and NLRP3 (f) at mean ± SEM. n = 3 in each group. Bars showed the levels of IL-1β (g) and IL-18 (h) by ELISA test in the hippocampus at mean ± SEM. n = 4 in each group. Significant differences were established by one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001