Fig. 3
From: Bruton’s tyrosine kinase drives neuroinflammation and anxiogenic behavior in mouse models of stress
Physical stress leads to induction of NLRP3 inflammasome and Caspase 1 activation in hippocampus and amygdala of mice. A Immunoblot analysis of NLRP3 and cleaved Caspase 1 from hippocampus homogenates. B The relative densitometry of NLRP3 immunoblots of samples from the hippocampus, which shows physical stress-induced higher levels of NLRP3 inflammasome in female mice in comparison to male mice. C Western blot analysis of NLRP3 and cleaved Caspase 1 in amygdala homogenates. D The relative densitometry analysis of NLRP3 immunoblots of amygdala samples. The analysis showed stressed female mice exhibited increased induction of NLRP3 inflammasome in comparison to male mice. Caspase 1 activity in the hippocampus E and amygdala (F), as measured in respective brain homogenates using Caspase 1 assay kit. Female mice subjected to physical stress showed significantly higher Caspase 1 activation relative to their male counterparts and control mice. All values are presented as mean with 95% CI (n = 6–17/group); ***p < 0.001, ns (not significant); two-way ANOVA followed by Bonferroni post hoc test