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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Argonaute-2 protects the neurovascular unit from damage caused by systemic inflammation

Fig. 3

Ago2 silencing reduces microglial inflammatory responses. LPS (LPS, 100 ng/ml) increased Ago2 intracellular levels in primary microglia, while the transfection with 0.05 µM of Ago2 siRNA reduced the intracellular levels of Ago2, even after LPS stimulation, measured by western blotting (a). Ago2 silencing was cytotoxic only in conjunction with LPS (b). Ago2-silenced microglial revealed lower levels of NO (c) and IL-6 (e), but not TNF-α (d), in an inflammatory environment, measured by ELISA. TRAF6 expression was reduced after LPS challenge but Ago2 silencing counteracted this effect, measured by western blotting (f). Data are expressed as the mean ± SEM of the indicated number of repeats and as a percentage relative to untreated controls (*p < 0.05, **p < 0.01; ***p < 0.001, compared to untreated controls; #p < 0.05, ##p < 0.01, ###p < 0.001, compared to LPS-activated cells; one-way ANOVA for all figures; in d, Student’s t test was used for the comparison between CTR and LPS). Ago2 argonaute-2, IL-6 interleukin-6, LPS lipopolysaccharide, NO nitric oxide, TNF-α tumor necrosis factor-alpha, TRAF6 TNF receptor-associated factor 6

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