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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Argonaute-2 protects the neurovascular unit from damage caused by systemic inflammation

Fig. 4

Ago2-restored endothelium normalizes microglia response. Schematic representation of the experimental setup (a). Endothelial cell-conditioned media (EC-CM) from cells exposed to LPS and treated with Ago2 (0.4 nM) ((LPS + Ago2 EC)-CM) reproduced the protective effect of EC-CM collected from healthy endothelial cells ((CTR EC)-CM), measured by LDH activity assay (b). Microglial cells exposed to (LPS + Ago2 EC)-CM presented CD11b levels similar to untreated cells (CTR) and cells exposed to (CTR EC)-CM, measured by western blotting, while (LPS EC)-CM had the same effect as the LPS treatment (c). EC-CM had no significant effect over IL-1β release (d), but (CTR EC)-CM and (LPS + Ago2 EC)-CM decreased NO below control levels (e). Data are expressed as the mean ± SEM of the indicated number of repeats and as a percentage relative to untreated controls (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, compared to untreated controls; #p < 0.05, ##p < 0.01, ###p < 0.001, ####p < 0.0001, compared to LPS-activated cells; §p < 0.05, §§§p < 0.001, §§§§p < 0.0001, compared to (CTR EC)-CM; $p < 0.05, $$$p < 0.001, $$$$p < 0.0001, compared to (LPS EC)-CM; one-way ANOVA; in d, Student’s t test was used for the comparison between CTR and LPS)). Ago2 argonaute-2, CD11b cluster of differentiation molecule 11b, EC-CM endothelial cell-conditioned media, IL-1β interleukin-1 beta, LPS lipopolysaccharide, NO nitric oxide

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