Fig. 2

cPLA₂α and misfolded SOD1 accumulates specifically in motor neurons of young pre-symptomatic SOD1^G93A mice. A Double staining (red) of motor neurons (ChAT), microglia (Iba-1) or astrocytes (GFAP) and misfolded SOD1 (B8H10, green) in the spinal cord sections of 6 weeks old pre-symptomatic SOD1^G93A mice. Scale bars = 20 μm. Shown a representative out of five mice. B Double staining (red) of motor neurons (ChAT), microglia (Iba-1) or astrocytes (GFAP) and cPLA₂α (green) in the spinal cord sections of 6 weeks old pre-symptomatic SOD1^G93A mice. Scale bars = 20 μm. Shown a representative out of five mice. C Double immunofluorescence staining of cPLA₂α (red) with misfolded SOD1 (B8H10, green) proteins in the spinal cord sections of control mice (WT) and 6 weeks old pre-symptomatic mutant SOD1^G93A mice. Scale bars = 100 μm. D The Pearson coefficient correlation (r) between cPLA₂α and misfolded SOD1 in the motor neurons of the intensity of cPLA₂α and misfolded SOD1 expressed in arbitrary units of immunostaining as presented in the representative results in C. Four filed in each of the 8 different mice analyzed