Fig. 4

Partial binding between cPLA₂α and mutant SOD1^G93A in motor neurons of 6 weeks old mutant SOD1^G93A mice. A Immunoprecipitation with antibody against cPLA₂α and Western Blot analysis for cPLA₂α and mutant SOD1 in the spinal cord lysate of control (WT) and mutant SOD1^G93A mice during the development of the disease shown by a representative immunoblot. Negative control (n.c). -without antibodies against cPLA₂α. B Motor performance on accelerating Rotarod test (n = 10 mice). The arrows show the immunoprecipitation analysis along the development of the disease. C Airyscan detector high resolution confocal microscopy images of double immunofluorescence staining of cPLA₂α (red) with misfolded SOD1 (B8H10, green) proteins in the spinal cord sections of 6 weeks old pre-symptomatic SOD1^G93A mouse. Scale bars = 20 μm. D The coupling of both proteins (cPLA₂α and misfolded SOD1) in the line shown by the arrow was analyzed and presented as intensity along the line. The ellipse shows an example of binding between both proteins